When ERCC members developed the RNA spike-in controls, they contributed RNA sequences designed to mimic, but not interfere with, mammalian RNA. The result of this collaborative effort was SRM 2374, a set of 96 DNA templates used to make ERCC RNA control molecules.
The erccdashboard is the first software tool of its kind designed to provide a standard turn-key solution for evaluating the technical performance of any gene expression experiment. This unique tool produces performance metrics that are independent from the type of measurement technology used for an experiment.
Selected publications related to the ERCC RNA controls
- Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak
Gire et al., Science, 2014
- Assessing technical performance in differential gene expression experiments with external spike-in RNA control ratio mixtures
Munro et al., Nature Communications, 2014